1. Biology Dictionary
  2. Scintillation Proximity Assay

Scintillation Proximity Assay闪烁近距离测定法

释义:

Scintillation Proximity Assay is used to measure the interaction strength between a ligand and its potential binding partners. When a labeled ligand binds to beads containing fluorescent groups, the binding amount of the radioactive-labeled ligand is measured using a scintillation counter. This method can also be used to estimate binding affinity, enzyme-catalyzed substrate cleavage, and modification of residues.

闪烁近距离测定法用于测量配体与其潜在结合伙伴的相互作用强度。标记配体与含荧光团的珠子结合时,通过闪烁计数器测量放射性标记配体的结合量。此方法还可用于估算结合亲和力、酶促底物裂解及修饰残基的滴定。

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