1. Biology Dictionary
  2. Split Luciferase Complementation Assay

Split Luciferase Complementation Assay裂解荧光素酶互补法

释义:

Split Luciferase Complementation Assay uses the C-terminal of luciferase protein fused with the target protein to study protein-protein interactions or functional recovery. The split luciferase regains its activity upon binding, and the luminescent signal is used for quantitative detection, widely applied in the study of receptor-ligand binding and signal transduction.

裂解荧光素酶互补法通过将荧光素酶蛋白的 C 末端与靶蛋白融合,用于研究蛋白质间的相互作用或功能恢复。裂解的荧光素酶在结合后恢复活性,通过发光信号定量检测,广泛用于研究受体-配体结合和信号转导。

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