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  2. Two Hybrid Bait and Prey Pooling Approach

Two Hybrid Bait and Prey Pooling Approach诱饵-猎物池化双杂交法

释义:

The Bait-Prey Pooling Two Hybrid Approach is used to screen for interaction partners between baits and preys, widely applied in constructing protein interaction maps and discovering drug targets. By pairing a single bait with a prey pool or pairing a bait pool with a single prey, this method requires cloning the bait and prey into two separate two-hybrid vectors, followed by grouped screening of the transformant collection. Positive two-hybrid clones are the interacting protein partners, providing a powerful tool for in-depth studies of protein networks.

诱饵-猎物池化双杂交法用于筛选诱饵和猎物之间的相互作用伙伴,广泛应用于蛋白质互作图谱的构建及药物靶点的发现。通过将单个诱饵与猎物池配对,或将诱饵池与单个猎物配对,此方法需将诱饵和猎物分别克隆到两个双杂交载体中,随后对转化子集合进行分组筛选。正向双杂交克隆即为相互作用的蛋白伙伴,从而为深入研究蛋白质网络提供了有力工具。

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