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  2. Two Hybrid Fragment Pooling Approach

Two Hybrid Fragment Pooling Approach双杂交片段池化法

释义:

The Two Hybrid Fragment Pooling Approach is used to identify the minimum functional regions required for protein interactions, mainly used in functional protein domain analysis and protein network construction. This method pairs a single bait with a random fragment prey pool, using degraded fragments to quickly locate the minimal protein region related to the interaction. Since multiple clones that encode overlapping protein regions can be identified, the initial screening visually shows the minimal domains required for the interaction.

双杂交片段池化法可用于鉴定蛋白相互作用所需的最小功能区域,主要用于功能蛋白域的解析和蛋白网络的构建。该方法通过将单个诱饵与随机片段猎物池配对,利用退化片段快速定位与相互作用相关的最小蛋白区域。由于可以识别编码重叠蛋白区域的多个克隆,初次筛选即可直观地显示相互作用所需的最小结构域。

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