2. 荧光染料
  3. 细胞染色分析
  4. 离子荧光探针
  5. 其他离子探针
  6. Bromothymol Blue sodium

Bromothymol Blue sodium  (Synonyms: 溴百里酚蓝钠盐；3',3''-二溴麝香草酚磺酰酞钠盐)

目录号: HY-D0012A
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Bromothymol Blue sodium salt 是一种 pH 指示剂，是测定弱酸弱碱时使用的一种指示剂，其酸性时为黄色，碱性时为蓝色。储存方式：避光。

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Bromothymol Blue sodium Chemical Structure

Bromothymol Blue sodium Chemical Structure

CAS No. : 34722-90-2

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Bromothymol Blue sodium 的其他形式现货产品:

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Bromothymol Blue sodium 相关抗体

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  • 生物活性

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生物活性

Bromothymol Blue sodium salt is a pH indicator. Storage: protect from light.

体外研究
(In Vitro)

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of Bromothymol Blue as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Bromothymol Blue sodium 相关抗体:
分子量

646.36

Formula

C27H27Br2NaO5S
CAS 号
性状

固体

中文名称

溴百里酚蓝钠盐；3',3''-二溴麝香草酚磺酰酞钠盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

纯度 & 产品资料
参考文献
Kinase Assay
[1]

For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress (DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL Bromothymol Blue (BTB) and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Bromothymol Blue sodium 相关分类

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Keywords:

Bromothymol Blue34722-90-2溴百里酚蓝钠盐3',3''-二溴麝香草酚磺酰酞钠盐OthersInhibitorinhibitorinhibit

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