Butaprost free acid

目录号: HY-120973: Data Sheet 产品使用指南技术支持

Butaprost free acid 是一种选择性前列腺素 E 受体 (EP2) 激动剂，对鼠类 EP2 受体的 EC₅₀ 值为 33 nM，K_i 值为 2.4 μM。Butaprost free acid 对鼠类 EP1，EP3 和 EP4 受体的活性较低。Butaprost free acid 通过阻碍 TGF-β/Smad2 信号传导来减轻纤维化。

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Butaprost free acid Chemical Structure

CAS No. : 215168-33-5

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规格	价格	是否有货	数量
500 μg	￥1818	3 - 4 周

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Other Forms of Butaprost free acid:

Butaprost In-stock
(S)-Butaprost free acid 询价

MCE 顾客使用本产品发表的 2 篇科研文献

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生物活性
纯度 & 产品资料
参考文献

生物活性

Butaprost free acid is a selective prostaglandin E receptor (EP2) agonist with an EC₅₀ of 33 nM and a K_i of 2.4 μM for murine EP2 receptor. Butaprost free acid is less activity against murine EP1, EP3 and EP4 receptors. Butaprost free acid attenuates fibrosis by hampering TGF-β/Smad2 signalling^[1]^[2]^[3].

体外研究
(In Vitro)

Butaprost (1-100 nM；0.5-24 小时) free acid 诱导 hEP2-HEK293/EBNA 细胞中 Nur77 mRNA 表达，上调大约五倍，且呈剂量和时间依赖性。Butaprost free acid 通过 PKC 通路上调 Nur77 基因表达^[1]。
Butaprost (50 μM；24 小时) free acid 降低 TGF-β 诱导的纤连蛋白 (FN) 表达、Smad2 磷酸化和上皮-间充质 Madin-Darby 犬肾 (MDCK) 细胞的转变^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK 293/EBNA cells stably expressing the human EP2 receptor (hEP2-HEK 293/EBNA cells).
Concentration:	1 nM, 10 nM, 100 nM
Incubation Time:	0.5 hours, 1 hours, 6 hours, 24 hours
Result:	Induced about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells.

体内研究
(In Vivo)

Butaprost (1-4 mg/kg；腹膜内注射；每天两次；持续 7 天) free acid 处理可减轻接受单侧输尿管梗阻手术的小鼠纤维化的发展，如 α-平滑肌基因和蛋白表达的减少所示肌动蛋白、纤连蛋白和胶原蛋白 1A1^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice (8 weeks of age; 21 g) bearing unilateral ureteral obstruction surgery^[2].
Dosage:	1 mg/kg, 2 mg/kg, 4 mg/kg
Administration:	Intraperitoneal injection; twice daily; for 7 days
Result:	Attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery.

分子量

394.54

Formula

C₂₃H₃₈O₅

CAS 号

215168-33-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

Data Sheet (551 KB) 产品使用指南 (1538 KB)

参考文献

[1]. Yanbin Liang, et al. Upregulation of orphan nuclear receptor Nur77 following PGF(2alpha), Bimatoprost, and Butaprost treatments. Essential role of a protein kinase C pathway involved in EP(2) receptor activated Nur77 gene transcription. Br J Pharmacol. 2004 Jun;142(4):737-48. [Content Brief]

[2]. Michael Schou Jensen, et al. Activation of the prostaglandin E 2 EP 2 receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices. Acta Physiol (Oxf). 2019 Sep;227(1):e13291. [Content Brief]

[3]. K Tani, et al. Design and synthesis of a highly selective EP2-receptor agonist. Bioorg Med Chem Lett. 2001 Aug 6;11(15):2025-8. [Content Brief]