CMP-sialic acid synthetase (NmCSS)  (Synonyms: CMP-唾液酸合成酶（NmCSS）)

CMP-sialic acid synthetase (NmCSS) is an essential enzyme involved in the biosynthesis of carbohydrates and glycoconjugates containing sialic acids. CMP-sialic acid synthetase (NmCSS) activates free Sia, converting it to CMP-Sia, which is the only donor substrate for all sialyltransferases^[1].

产品信息
该酶用于活化唾液酸形成CMP-唾液酸，作为糖基转移酶转移唾液酸的供体，可以作唾液酸糖转移的工具酶。本品利用大肠杆菌重组表达，C-terminal His Tag，分子量26 kDa，储存缓冲液组分为20 mM Tris-HCl , pH 8.0, 5% glycerol。

使用说明
1.按照反应体系加入底物唾液酸和 CTP，一般控制浓度 100 mM 以下，反应体系中可适当加入缓冲盐 (如 Tris-HCl)；
2.在反应体系中加入5 mM MgCl2，混匀后，调节 pH 为7.5-8.5，加入适量酶 (根据反应需求)。
3.将样品置于 37℃ 孵育30-60 min。推荐反应缓冲是Tris-HCl (pH 8.0)，多数常见的生物缓冲也适用，比如 Tris 或 PBS。

注意事项
体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀，加入Inorganic pyrophosphatase, Saccharomyces cerevisiae (HY-P2992) 可以增加转化率。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9067-82-7

液体

Colorless to light yellow

CMP-唾液酸合成酶（NmCSS）

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wu D, et, al. A point-mutation in the C-domain of CMP-sialic acid synthetase leads to lethality of medaka due to protein insolubility. Sci Rep. 2021 Dec 1;11(1):23211.  [Content Brief]