Creatininase, Microorganism  (Synonyms: Creatinine amidohydrolase; CAH)

Creatininase, Microorganism (Creatinine amidohydrolase; CAH), namely creatinine amidohydrolase, from Pseudomonas putida, is a homohexameric enzyme commonly used in biochemical research. Creatininase acts on carbon-nitrogen bonds other than peptide bonds, and can catalyze the hydrolysis of creatinine to creatine, which can then be metabolized by creatinase to urea and sarcosine^[1].

产品信息
分子量：29 kDa (SDS-PAGE)
等电点：5.3
Km 值：5.0×10^-2 M (Creatinine)，8.0×10^-2 M (Creatine)
抑制剂：Hg²⁺，Cu²⁺，Fe³⁺
最适 pH：7.0-8.0
最适温度：65℃
pH 稳定性：pH 5.5-10.0 (25℃,16 h)
热稳定性：65℃ 以下稳定 (pH 8.0,30 min)

使用说明
可使用纯水（50 mg/mL 浓度以下）溶解酶，或根据具体实验参考文献 buffer 溶解。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9025-13-2

固体

White to off-white

肌酐酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Barbara Beuth, et al. Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution. J Mol Biol. 2003 Sep 5;332(1):287-301.  [Content Brief]