DCDAPH 

DCDAPH (Compound 2c) is a novel smart NIRF probe for detection of β-amyloid (Aβ) plaques (λ_ex/λ_em=597/665 nm in PBS). DCDAPH shows high affinity for Aβ aggregates (K_i=37 nM, K_d=27 nM). DCDAPH shows good blood brain barrier permeation and can meet most of the requirements for the detection of Aβ plaques both in vitro and in vivo^[1].

DCDAPH (0-10 μM; 24 h) treatment shows no toxicity to human neuronal cells^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fluorescent Staining of DCDAPH to Aβ Plaques in Mouse Brain^[1].
1. Mouse should be i.v. injected with DCDAPH (0.4 mg/kg, 20% DMSO (HY-Y0320), 80% propylene glycol (HY-Y0921), 50 μL), and sacrificed at 30 min after injection.
2. The brains should be excised, embedded in optimum cutting temperature compound (OCT), and frozen in powdered dry ice immediately.
3. Frozen sections of 20 μm should be cut.
4. Fluorescent observation (Cy5 filter set).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

249.31

C₁₆H₁₅N₃

125113-96-4

665

597

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mengchao Cui, et al. Smart near-infrared fluorescence probes with donor-acceptor structure for in vivo detection of β-amyloid deposits. J Am Chem Soc. 2014 Mar 5;136(9):3388-94.  [Content Brief]