Direct Red 80  (Synonyms: 天狼猩红; Sirius Red)

Direct Red 80 (Sirius Red) is a polyazo dye used principally in staining methods for collagen and amyloid. Direct Red 80 does not release benzidine upon degradation and is safer than many traditional direct dyes^[1].

共聚焦激光显微镜 (CLM) 也用于评估 Direct Red 80 染色后的胶原蛋白生成和分布。Direct Red 80 对不同类型的胶原蛋白没有特异性。样品在 dH₂O 中清洗，用 0.2% phosphomolybdic acid (PMA) 溶液预处理 40 分钟，然后在由 0.1% Direct Red 80 稀释在饱和苦味酸水溶液中的溶液中孵育 30 分钟。最后，每个样品在 0.01 M HCl 中清洗 2 分钟。PMA 用于使细胞质无色，HCl 用于去除非特异性染色^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1373.07

C₄₅H₂₆N₁₀Na₆O₂₁S₆

2610-10-8

固体

Brown to reddish brown

天狼猩红

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Dapson RW, et al. Certification procedures for sirius red F3B (CI 35780, Direct red 80). Biotech Histochem. 2011;86(3):133-139.  [Content Brief]