Elastase, Human leukocytes  (Synonyms: 弹性蛋白酶来源于人类白血球)

Elastase, Human leukocytes is a serine protease present in the nitrogen-loving granules of neutrophils. Elastase, Human leukocytes potential substrates include almost all components of the extracellular matrix, as well as a variety of proteins such as coagulation factors, complement, immunoglobulins and cytokines. It has a strong proteolytic function and participates in the pathogenesis of inflammatory tissue damage^[1][2].

Elastase, Human leukocytes 水解 IL-36Ra 实验^[2]
(1) 细胞在无血清培养基或 Opti-MEM 培养基中生长，以避免任何活性蛋白酶的抑制。分离后立即用 100 ng/mL PMA 在 37℃ 下刺激中性粒细胞 1 小时，然后离心取出细胞并收集上清。
(2) 1-2 μg SUMO 标记的 IL-36Ra 与原代细胞上清液，在有或没有蛋白酶抑制剂 (IAA 50 μM、EDTA 500 μM、PMSF 1 mM、1x Roche 蛋白酶抑制剂混合物、α1 抗胰蛋白酶 1 mg/mL) 的条件下，在 37℃ 孵育 1 小时。
(3) 用 Elastase, Human leukocytes 在 37°C 下孵育 5 小时。
(4) 通过 SDS-PAGE 或 Western Blotting 使用单克隆抗 IL-36Ra 抗体进行分析。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9004-06-2

固体

White to off-white

弹性蛋白酶来源于人类白血球

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. W L Lee, et al. Leukocyte elastase: physiological functions and role in acute lung injury. Am J Respir Crit Care Med. 2001 Sep 1;164(5):896-904.  [Content Brief]

  [2]. Tom Macleod, et al. Neutrophil Elastase-mediated proteolysis activates the anti-inflammatory cytokine IL-36 Receptor antagonist. Sci Rep. 2016 Apr 22:6:24880.  [Content Brief]