Endo-β-Galactosidase  (Synonyms: 内切-β-半乳糖苷酶)

Endo-β-Galactosidase catalyzes the hydrolysis of internal β1-4 galactose linkages in unbranched, repeating poly-N-acetyllactosamine ([GlcNAc- (1-3)Gal- (1-4)]_n) structures^[1].

others

产品信息
本品利用大肠杆菌重组表达，N-terminal His-tag，分子量 36 kDa，缓冲液组分 20 mM Tris-HCl, pH 7.5, 500 mM NaCl ,10% glycerol，可水解聚 N -乙酰乳胺结构中低聚糖（LNnT）内部的 β-半乳糖苷键。

使用说明
1. 在反应体系中依次加入底物，50 mM 醋酸钠, pH 5.8，加入 5 mM CaCl₂ 混匀，加入适量内切-β-半乳糖苷酶。 2. 将样品置于 37℃ 孵育 2-4h 或者过夜。

注意事项
体系放大时孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

52720-51-1

液体

Colorless to light yellow

内切-β-半乳糖苷酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. P Scudder, et al. Isolation and characterization of an endo-beta-galactosidase from Bacteroides fragilis.Biochem J. 1983 Aug 1; 213(2): 485–494.