FD-1080 

FD-1080 is a fluorophore with both excitation and emission in the NIR-II region (Ex=1064 nm, Em=1080 nm). FD-1080 can be used for in vivo imaging^[1].

1. FD-1080 工作液的配制
1.1 制备储存液
用 DMSO 稀释 FD-1080 配制 10 mM 的 FD-1080。
注：FD-1080 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。
1.2 工作液的配制
用预热好的PBS 稀释储存液，配制成 2-10 μM 的 FD-1080 工作液。0.2 μm 滤膜过滤除菌。
注：请根据实际情况调整 FD-1080 工作液浓度，且现用现配。
2. 细胞实验
2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。
贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。
2.2 加入 1 mL FD-1080 工作液，室温孵育 10-30 分钟。
2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。
2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。
3. 活体注射
3.1 将 200 μL 80 μM FD-1080工作液静脉注射至小鼠体内，10-20 分钟后进行体内成像分析。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

The 1064 nm NIR-II excitation of FD-1080 is demonstrated with the high tissue penetration depth and superior imaging resolution compared to NIR excitation from 650 nm to 980 nm. Deeptissue and high-resolution in vivo imaging for the left hindlimb vasculature, abdomen, and brain vessels was realized, allowing penetration through intact skin, tissue, and skull. FD-1080 also quantifying the respiratory rate based on the dynamic imaging of respiratory craniocaudal motion of the liver for the awake and anaesthetized mouse^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

765.31

C₄₀H₃₈ClN₂NaO₆S₂

1151666-58-8

固体

Brown to black

1000

985

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Li B, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487.  [Content Brief]

  [2]. Benhao Li, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487.  [Content Brief]