FDGlcU 

FDGlcU can be used as a fluorescent probe for non-invasively image with a high level of fluorescent activity. FDGlcU is non-fluorescent when the fluorescein is conjugated with two mono-glucuronides (Ex/Em=480/514 nm)^[1].

FDGlcU (0.5 μg/mL; 12 h) shows enzymatic activity and inhibits βG activity in live bacteria^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

FDGlcU (7.3 μM/kg) can be used for in vivo time-lapse imaging of bacterial βG activity^[1].
FDGlcU can be used for real-time imaging in vivo^[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Whole-body imaging for intestinal βG activity^[1]:
1. On the day of imaging, gavage mice with 50 μL of DDW, FDGlcU (7.3 μM/kg), and fluorescein (7.3 μM/kg).
2. Anesthetize mice with isoflurane and whole-body optical images using an IVIS spectrum optical imaging system with a GFP filter set.
3. In biodistribution study, mice are sacrificed 3 hour after gavaged with FDGlcU or fluorescein (λex =480 nm, λem =514 nm).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

893.63

C₃₉H₂₈F₅NO₁₈

209540-67-0

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chen M, et al. Real-time imaging of intestinal bacterial β-glucuronidase activity by hydrolysis of a fluorescent probe. Sci Rep. 2017 Jun 9;7(1):3142.  [Content Brief]