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Gelatins  (Synonyms: 明胶)

目录号: HY-Y1365 纯度: ≥98.0%
COA 产品使用指南

Gelatins 是一种源自胶原蛋白部分水解的无毒、无致癌、可生物降解、不易刺激的天然聚合物,因其强大的液体吸收和溶胀能力具有优异的止血性能,可以作为金属纳米颗粒的还原、生长和稳定性的基质材料,也可以用于肿瘤细胞培养和肿瘤治疗。

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Gelatins Chemical Structure

Gelatins Chemical Structure

CAS No. : 9000-70-8

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Gelatins is a non-toxic, non carcinogenic, biodegradable, and non irritating natural polymer derived from partial hydrolysis of collagen. Due to its strong liquid absorption and swelling ability, Gelatins has excellent hemostatic properties and can be used as a matrix material for the reduction, growth, and stability of metal nanoparticles. Gelatins can also be used for tumor cell culture and tumor therapy[1][2][3].

体外研究
(In Vitro)

Gelatins (6-12 %, 5 天) 通过形成 gelatin@NWF 衍生物作为长期储存支架促进形成前列腺肿瘤细胞球体,用于培养肿瘤细胞[3]
Gelatins (0-200 μg/mL, 24 h) 可以增强 HaCaT 细胞的细胞粘附、细胞生长和伤口愈合,并保护 HaCaT 细胞免受H2O2诱导的细胞损伤[5]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3].

Cell Line: Human prostate cancer cell DU145
Concentration: 6-12 %
Incubation Time: 5 days
Result: Supported more cell growth than pure non-woven fabric (NWF) scaffolds

Cell Viability Assay[5].

Cell Line: Human keratinocyte-derived (HaCaT) cells
Concentration: 0-200 μg/mL
Incubation Time: 24 h
Result: Increased cell growth percentage in the concentration range of 25-75 μg/mL, but decreased cell growth percentage in the concentration range of 100-200 μg/mL.
体内研究
(In Vivo)

Gelatins (20 μg/kg,腹腔注射) 通过形成金纳米颗粒/明胶纳米复合材料对 Wistar 大鼠脊髓损伤具有良好的运动恢复改善作用[1]
Gelatins 在与生物相容性二醛淀粉 (DAS) 交联合成胺化明胶海绵后对大鼠股动脉损伤具有优异的止血作用[2]
Gelatins (10 mg DOX/kg; 每 2 天 1 次; 14 天; 静脉注射) 通过 PEG 修饰形成 pH/酶响应复合物可以特异性递送阿霉素 (DOX) (HY-15142A),在 S180 异位肿瘤小鼠中具有抗肿瘤活性[4]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Traumatic spinal cord injury Wistar rat model[1].
Dosage: 20 μg/kg Au NPs/Gelatin nanocomposite
Administration: Intraperitoneal injection (i.p.)
Result: Improved hind limb function, reduced cavity area, increased the number of ventral motor neurons, and demonstrated higher BBB scores and lower delayed responses in sensory tests.
Animal Model: SD female rat femoral injury model[2].
Dosage: A pre-weighed hemostatic sponge
Administration: Apply to the wound
Result: Reduced hemostasis time and blood loss.
Animal Model: Female ICR mouse model of ectopic transplantation of mouse sarcoma cell line S180[4].
Dosage: 10 mg DOX/kg
Administration: Intravenous injection (i.v.); once every two days; 14 days
Result: Reduced tumor volume, prevented tumor cell spread, and lowered cardiac toxicity.
CAS 号
性状

固体

颜色

White to yellow

中文名称

明胶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验: 

H2O 中的溶解度 : 50 mg/mL (超声助溶)

DMSO 中的溶解度 : 4.55 mg/mL (超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验:

以下溶解方案,请直接配制工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 10 mg/mL; 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥98.0%

参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Gelatins
目录号:
HY-Y1365
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