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IR 813 tosylate 

目录号: HY-W248594 纯度: 99.47%
COA 产品使用指南

IR 813 tosylate 是一种近红外 (NIR) 荧光染料 (λex=815 nm,λem=840 nm),可用于对小鼠的局部淋巴结进行可视化。

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IR 813 tosylate Chemical Structure

IR 813 tosylate Chemical Structure

CAS No. : 134127-48-3

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (0.1 - 0.2 mg)   Apply now  
10 mM * 1 mL in DMSO ¥308
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250 mg ¥280
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500 mg ¥440
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1 g ¥680
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10 g   询价  

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Customer Review

Other Forms of IR 813 tosylate:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

IR 813 tosylate is a near-infrared (NIR) fluorescent dyeex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice[1].

体外研究
(In Vitro)

IR 813 (17.3 μM, 2 h) tosylate induces a 31.4% hemolysis in red blood cells[1].
IR 813 (5.9 μM, 24 h) tosylate induces 50% cell death in MRC-5 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

IR 813 tosylate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

755.41

Formula

C47H47ClN2O3S

CAS 号
性状

固体

颜色

Light brown to brown

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 62.5 mg/mL (82.74 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3238 mL 6.6189 mL 13.2378 mL
5 mM 0.2648 mL 1.3238 mL 2.6476 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3238 mL 6.6189 mL 13.2378 mL 33.0946 mL
5 mM 0.2648 mL 1.3238 mL 2.6476 mL 6.6189 mL
10 mM 0.1324 mL 0.6619 mL 1.3238 mL 3.3095 mL
15 mM 0.0883 mL 0.4413 mL 0.8825 mL 2.2063 mL
20 mM 0.0662 mL 0.3309 mL 0.6619 mL 1.6547 mL
25 mM 0.0530 mL 0.2648 mL 0.5295 mL 1.3238 mL
30 mM 0.0441 mL 0.2206 mL 0.4413 mL 1.1032 mL
40 mM 0.0331 mL 0.1655 mL 0.3309 mL 0.8274 mL
50 mM 0.0265 mL 0.1324 mL 0.2648 mL 0.6619 mL
60 mM 0.0221 mL 0.1103 mL 0.2206 mL 0.5516 mL
80 mM 0.0165 mL 0.0827 mL 0.1655 mL 0.4137 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
IR 813 tosylate
目录号:
HY-W248594
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