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  3. L-Ascorbic acid 2-phosphate

L-Ascorbic acid 2-phosphate  (Synonyms: 维生素C磷酸酯; 2-Phospho-L-ascorbic acid)

目录号: HY-103701 含量: 37.4%
COA 产品使用指南

L-ascorbic acid 2-phosphate 是一种长效的维生素 C 衍生物 (vitamin C derivative),可以刺激胶原蛋白的表达和形成。L-ascorbic acid 2-phosphate 可以作为培养基补充人脂肪干细胞 (hASCs) 的成骨分化。L-ascorbic acid 2-phosphate 增加成骨细胞分化过程中 hASC 中的碱性磷酸酶 (ALP) 活性和 runx2A 的表达。

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L-Ascorbic acid 2-phosphate Chemical Structure

L-Ascorbic acid 2-phosphate Chemical Structure

CAS No. : 23313-12-4

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) is a long-acting vitamin C derivative that can stimulate collagen formation and expression[1]. L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation[2][3].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc)  achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect [1].
L-Ascorbic acid 2-phosphate (50 µM-250 µM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Human corneal endothelial cells (HCECs) 
Concentration: 0.1 mM; 0.3 mM; 1.5 mM
Incubation Time: 2 to 3 weeks with medium exchange every 2 to 3 days
Result: Stimulated HCEC cells growth.
分子量

256.10

Formula

C6H9O9P

CAS 号
性状

固体

颜色

Off-white to yellow

中文名称

维生素C磷酸酯

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
细胞实验: 

1 M HCL 中的溶解度 : 25 mg/mL (97.62 mM; 超声助溶)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.9047 mL 19.5236 mL 39.0472 mL
5 mM 0.7809 mL 3.9047 mL 7.8094 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 37.4%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
1 M HCL 1 mM 3.9047 mL 19.5236 mL 39.0472 mL 97.6181 mL
5 mM 0.7809 mL 3.9047 mL 7.8094 mL 19.5236 mL
10 mM 0.3905 mL 1.9524 mL 3.9047 mL 9.7618 mL
15 mM 0.2603 mL 1.3016 mL 2.6031 mL 6.5079 mL
20 mM 0.1952 mL 0.9762 mL 1.9524 mL 4.8809 mL
25 mM 0.1562 mL 0.7809 mL 1.5619 mL 3.9047 mL
30 mM 0.1302 mL 0.6508 mL 1.3016 mL 3.2539 mL
40 mM 0.0976 mL 0.4881 mL 0.9762 mL 2.4405 mL
50 mM 0.0781 mL 0.3905 mL 0.7809 mL 1.9524 mL
60 mM 0.0651 mL 0.3254 mL 0.6508 mL 1.6270 mL
80 mM 0.0488 mL 0.2440 mL 0.4881 mL 1.2202 mL
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产品名称:
L-Ascorbic acid 2-phosphate
目录号:
HY-103701
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