2. Academic Validation
  3. Novel inhibitors of bacterial cytokinesis identified by a cell-based antibiotic screening assay

Novel inhibitors of bacterial cytokinesis identified by a cell-based antibiotic screening assay

  • J Biol Chem. 2005 Dec 2;280(48):39709-15. doi: 10.1074/jbc.M506741200.
Neil R Stokes 1,  Jörg Sievers,  Stephanie Barker,  James M Bennett,  David R Brown,  Ian Collins,  Veronica M Errington,  David Foulger,  Michelle Hall,  Rowena Halsey,  Hazel Johnson,  Valerie Rose,  Helena B Thomaides,  David J Haydon,  Lloyd G Czaplewski,  Jeff Errington
Affiliations

Affiliation

  • 1 Prolysis Ltd., Oxford University Begbroke Science Park, Oxfordshire, OX5 1PF, United Kingdom.
PMID: 16174771 DOI: 10.1074/jbc.M506741200
Abstract

The continuous emergence of Antibiotic resistance demands that novel classes of Antibiotics continue to be developed. The division machinery of bacteria is an attractive target because it comprises seven or more essential proteins that are conserved almost throughout the bacteria but are absent from humans. We describe the development of a cell-based assay for inhibitors of cell division and its use to isolate a new inhibitor of FtsZ protein, a key player in the division machinery. Biochemical, cytological, and genetic data are presented that demonstrate that FtsZ is the specific target for the compound. We also describe the effects of more potent analogues of the original hit compound that act on important pathogens, again at the level of cell division. The assay and the compounds have the potential to provide novel Antibiotics with no pool of pre-existing resistance. They have provided new insight into cytokinesis in bacteria and offer important reagents for further studies of the cell division machinery.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z