1. Academic Validation
  2. Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice

Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice

  • J Allergy Clin Immunol. 2007 Jun;119(6):1522-8. doi: 10.1016/j.jaci.2007.01.034.
Shih-Chang Hsu 1 Tsung-Hsien Tsai Hirokazu Kawasaki Chien-Ho Chen Beverly Plunkett Reiko T Lee Yuan C Lee Shau-Ku Huang
Affiliations

Affiliation

  • 1 Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA. skhuang@jhmi.edu
Abstract

Background: Glycoproteins containing Lewis-x (Le(x)) trisaccharides are often associated with the host's adaptive T(H)2-type immunity, but the mechanisms underlying the T(H)2-biased response are at present unclear.

Objective: The modulatory effect of Le(x) or its glycoconjugates on IgE/T(H)2 responses was investigated.

Methods: The levels of serum Antibodies and cytokines were analyzed by means of ELISA, RT-PCR, or both.

Results: In C3H mice Le(x) coupled with BSA (Le(x)-BSA) elicited higher levels of specific IgE and IgG1, but not IgG2A, which were associated with increased levels of splenic T(H)2 cytokines when compared with those seen in BSA-sensitized mice. In BALB/c mice sensitized with Le(x)-BSA or Le(x) mixed with ovalbumin, significantly increased levels of specific IgE and IgG2a Antibodies were found concomitant with reduced levels of serum IL-12p70. These effects were attenuated in IL-12-deficient BALB/c mice. Le(x) and an isomer, Le(y), but not Other isomers, inhibited the production of LPS-induced IL-12p70, associated with a significant reduction of nuclear NF-kappaB, in bone marrow-derived dendritic cells from BALB/c mice, suggesting that Le(x)-induced suppression of IL-12p70 results in an enhanced T(H)2 response. The addition of mannan, a known ligand for dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin, abrogated the suppressive effect of Le(x) trisaccharides.

Conclusion: These results provide evidence for a potential role of Le(x) trisaccharides in shaping the immune responses through, at least in part, its suppressive effect on IL-12p70 production. Considering the relative ubiquity of glycoproteins with Le(x) or similar oligosaccharides, including plant-derived (or food-derived) allergens, these findings might have a broad implication.

Clinical implications: The Adjuvant activity of Le(x) trisaccharides might aid in vaccine design and might be important in determining the allergenicity of proteins containing this or Other similar structures.

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