2. Academic Validation
  3. PI(3,4,5)P3 Interactome

PI(3,4,5)P3 Interactome

  • J Proteome Res. 2009 Jul;8(7):3712-26. doi: 10.1021/pr900320a.
Bruno Catimel 1,  Meng-Xin Yin,  Christine Schieber,  Melanie Condron,  Heather Patsiouras,  Jenny Catimel,  Diane E J E Robinson,  Leon S-M Wong,  Edouard C Nice,  Andrew B Holmes,  Antony W Burgess
Affiliations

Affiliation

  • 1 Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, and School of Chemistry, Bio21 Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Bruno.catimel@ludwig.edu.au
PMID: 19463016 DOI: 10.1021/pr900320a
Abstract

Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon Cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.

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