2. Academic Validation
  3. Elucidation of a structural basis for the inhibitor-driven, p62 (SQSTM1)-dependent intracellular redistribution of cAMP phosphodiesterase-4A4 (PDE4A4)

Elucidation of a structural basis for the inhibitor-driven, p62 (SQSTM1)-dependent intracellular redistribution of cAMP phosphodiesterase-4A4 (PDE4A4)

  • J Med Chem. 2011 May 12;54(9):3331-47. doi: 10.1021/jm200070e.
Jonathan P Day 1,  Barbara Lindsay,  Tracy Riddell,  Zhong Jiang,  Robert W Allcock,  Achamma Abraham,  Sebastian Sookup,  Frank Christian,  Jana Bogum,  Elisabeth K Martin,  Robert L Rae,  Diana Anthony,  Georgina M Rosair,  Daniel M Houslay,  Elaine Huston,  George S Baillie,  Enno Klussmann,  Miles D Houslay,  David R Adams
Affiliations

Affiliation

  • 1 Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, U.K.
PMID: 21456625 DOI: 10.1021/jm200070e
Abstract

A survey of PDE4 inhibitors reveals that some compounds trigger intracellular aggregation of PDE4A4 into accretion foci through association with the ubiquitin-binding scaffold protein p62 (SQSTM1). We show that this effect is driven by inhibitor occupancy of the catalytic pocket and stabilization of a "capped state" in which a sequence within the enzyme's upstream conserved region 2 (UCR2) module folds across the catalytic pocket. Only certain inhibitors cause PDE4A4 foci formation, and the structural features responsible for driving the process are defined. Switching to the UCR2-capped state induces conformational transition in the enzyme's regulatory N-terminal portion, facilitating protein association events responsible for reversible aggregate assembly. PDE4-selective inhibitors able to trigger relocalization of PDE4A4 into foci can therefore be expected to exert actions on cells that extend beyond simple inhibition of PDE4 catalytic activity and that may arise from reconfiguring the enzyme's protein association partnerships.

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