1. Academic Validation
  2. [Mouse melanoma cell line B16F10-derived conditioned medium inhibits sodium L-ascorbate-induced B16F10 cell apoptosis]

[Mouse melanoma cell line B16F10-derived conditioned medium inhibits sodium L-ascorbate-induced B16F10 cell apoptosis]

  • Nan Fang Yi Ke Da Xue Xue Bao. 2012 Feb;32(2):146-50.
Xuhui Yang 1 Tian Xia Weihua Yu Xiaofang Lu Peng Xiang Feng He
Affiliations

Affiliation

  • 1 Guangdong Women and Children's Hospital, Guangzhou, China. yxhshy52@163.com
PMID: 22381745
Abstract

Objective: To investigate the effect of mouse melanoma cell line B16F10-derived conditioned medium on the Apoptosis of B16F10 cells.

Methods: B16F10 cells were cultured in high-glucose DMEM in the presence of 10% Fetal Bovine Serum, and upon cell confluence, the growth medium was replaced with serum-free high-glucose DMEM. After 8 h, the medium was collected and infiltrated to serve as the conditioned medium. B16F10 cells cultured in normal growth medium or the conditioned medium were exposed to 10 mmol/L sodium L-ascorbate, and the cell Apoptosis was analyzed. The ingredients in the conditioned medium with relative molecular mass less or more than 5 000 were extracted to assess their effect on sodium L-ascorbate-induced cell Apoptosis.

Results: The conditioned medium for B16F10 cells significantly inhibited cell Apoptosis induced by sodium L-ascorbate, and the effective ingredients in the medium showed a relative molecular mass below 5,000.

Conclusion: Mouse melanoma cell line B16F10-derived conditioned medium can suppress sodium L-ascorbate- induced Apoptosis of B16F10 cells, and the ingredients with relative molecular mass less than 5 000 are responsible for this effect.

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