1. Academic Validation
  2. Charybdotoxin and noxiustoxin, two homologous peptide inhibitors of the K+ (Ca2+) channel

Charybdotoxin and noxiustoxin, two homologous peptide inhibitors of the K+ (Ca2+) channel

  • FEBS Lett. 1988 Jan 4;226(2):280-4. doi: 10.1016/0014-5793(88)81439-x.
H H Valdivia 1 J S Smith B M Martin R Coronado L D Possani
Affiliations

Affiliation

  • 1 Department of Physiology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030.
Abstract

We show that noxiustoxin (NTX), like charybdotoxin (CTX) described by Others, affects Ca2+-activated K+ channels of skeletal muscle (K+(Ca2+) channels). Chemical characterization of CTX shows that it is similar to NTX. Although the amino-terminal amino acid of CTX is not readily available, the molecule was partially sequenced after CNBr cleavage. A decapeptide corresponding to the C-terminal region of NTX shows 60% homology to that of CTX, maintaining the cysteine residues at the same positions. While CTX blocks the K+ (Ca2+) channels with a Kd of 1-3 nM, for NTX it is approx. 450 nM. Both Peptides can interact simultaneously with the same channel. NTX and CTX promise to be good tools for channel isolation.

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