2. Academic Validation
  3. Amorfrutin A inhibits TNF-α-induced NF-κB activation and NF-κB-regulated target gene products

Amorfrutin A inhibits TNF-α-induced NF-κB activation and NF-κB-regulated target gene products

  • Int Immunopharmacol. 2014 Jul;21(1):56-62. doi: 10.1016/j.intimp.2014.04.016.
Hui Shi 1,  Juan Ma 2,  Chunliu Mi 1,  Jing Li 1,  Fei Wang 2,  Jung Joon Lee 3,  Xuejun Jin 4
Affiliations

Affiliations

  • 1 Molecular Cancer Research Center, Yanbian University, Yanji 133002, Jilin Province, China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of education, Department of Chemistry, Yanbian University, Yanji 133002, Jilin Province, China.
  • 2 Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of education, College of Pharmacy, Yanbian University, Yanji 133002, Jilin Province, China; Molecular Cancer Research Center, Yanbian University, Yanji 133002, Jilin Province, China.
  • 3 Molecular Cancer Research Center, Yanbian University, Yanji 133002, Jilin Province, China. Electronic address: jjlee0908@gmail.com.
  • 4 Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of education, College of Pharmacy, Yanbian University, Yanji 133002, Jilin Province, China; Molecular Cancer Research Center, Yanbian University, Yanji 133002, Jilin Province, China. Electronic address: xjjin@ybu.edu.cn.
PMID: 24785328 DOI: 10.1016/j.intimp.2014.04.016
Abstract

The nuclear factor-κB (NF-κB) transcription factors control many physiological processes including inflammation, immunity, Apoptosis, and angiogenesis. In our search for NF-κB inhibitors from natural resources, we identified amorfrutin A as an inhibitor of NF-κB activation from the fruits of Amorpha fruticosa L. In present study, this compound significantly inhibited the TNF-α-induced expression of NF-κB reporter gene. Further analysis revealed that amorfrutin A was a potent inhibitor of NF-κB activation by the suppression of TNF-α-induced inhibitor of κBα (IκBα) degradation, p65 nuclear translocation, and DNA-binding activity of NF-κB. We also demonstrated that pretreatment of cells with this compound prevented the TNF-α-induced expression of NF-κB target genes, such as antiapoptosis (cIAP-1 and FLIP), proliferation (COX-2 and cyclinD1), invasion (MMP-9), angiogenesis (VEGF), and major inflammatory cytokines (TNF-α, IL-8, and MCP1). Furthermore, our results suggest that amorfrutin A potentiates TNF-α-induced Apoptosis. Taken together, amorfrutin A could be a valuable candidate for the intervention of NF-κB-dependent pathological conditions such as inflammation.

Keywords

Amorfrutin A; Apoptosis; Inflammation; IκBα; Nuclear factor-κB (NF-κB).

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z