2. Academic Validation
  3. Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

  • J Agric Food Chem. 2015 Sep 9;63(35):7862-72. doi: 10.1021/acs.jafc.5b02697.
Alexis V Nathanail 1,  Elisabeth Varga 2,  Jacqueline Meng-Reiterer 2 3,  Christoph Bueschl 2,  Herbert Michlmayr 4,  Alexandra Malachova 2,  Philipp Fruhmann 5,  Marika Jestoi 6,  Kimmo Peltonen 7,  Gerhard Adam 4,  Marc Lemmens 3,  Rainer Schuhmacher 2,  Franz Berthiller 2
Affiliations

Affiliations

  • 1 Chemistry and Toxicology Unit, Research and Laboratory Department, Finnish Food Safety Authority (Evira) , Mustialankatu 3, 00790 Helsinki, Finland.
  • 2 Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad Lorenz Str. 20, 3430 Tulln, Austria.
  • 3 Institute for Biotechnology in Plant Production, IFA-Tulln, BOKU , Konrad Lorenz Str. 20, 3430 Tulln, Austria.
  • 4 Department of Applied Genetics and Cell Biology, BOKU , Konrad Lorenz Str. 24, 3430 Tulln, Austria.
  • 5 Institute of Applied Synthetic Chemistry, Vienna University of Technology , Getreidemarkt 9/163, 1060 Vienna, Austria.
  • 6 Product Safety Unit, Control Department, Finnish Food Safety Authority (Evira) , Mustialankatu 3, 00790 Helsinki, Finland.
  • 7 Finnish Safety and Chemicals Agency (Tukes) , Opastinsilta 12 B, 00521 Helsinki, Finland.
PMID: 26278508 DOI: 10.1021/acs.jafc.5b02697
Abstract

To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well as acetylation and deacetylation products in wheat was obtained for the first time. To monitor the kinetics of metabolite formation, a time course experiment was conducted involving the Fusarium head blight susceptible variety Remus and the resistant cultivar CM-82036. Biotransformation reactions were observed already at the earliest tested time point (6 h after treatment), and formed metabolites showed different kinetic profiles. After ripening, less than 15% of the toxins added to the Plants were determined to be unmetabolized.

Keywords

cereals; liquid chromatography−high resolution mass spectrometry; masked mycotoxins; metabolomics; stable isotopes; trichothecenes.

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