2. Academic Validation
  3. Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis

Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis

  • Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):E41-50. doi: 10.1073/pnas.1521248112.
Chunhua Zhang 1,  Michelle Q Brown 1,  Wilhelmina van de Ven 1,  Zhi-Min Zhang 2,  Bin Wu 3,  Michael C Young 4,  Lukáš Synek 5,  Dan Borchardt 4,  Reed Harrison 6,  Songqin Pan 1,  Nan Luo 1,  Yu-Ming M Huang 4,  Yoo-Jin Ghang 4,  Nolan Ung 1,  Ruixi Li 1,  Jonathan Isley 7,  Dimitrios Morikis 6,  Jikui Song 2,  Wei Guo 3,  Richard J Hooley 4,  Chia-En A Chang 4,  Zhenbiao Yang 1,  Viktor Zarsky 8,  Gloria K Muday 7,  Glenn R Hicks 1,  Natasha V Raikhel 9
Affiliations

Affiliations

  • 1 Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521;
  • 2 Department of Biochemistry, University of California, Riverside, CA 92521;
  • 3 Department of Biology, University of Pennsylvania, Philadelphia, PA 19104;
  • 4 Department of Chemistry, University of California, Riverside, CA 92521;
  • 5 Institute of Experimental Botany, Academy of Sciences, 165 02 Prague 6, Czech Republic;
  • 6 Department of Bioengineering, University of California, Riverside, CA, 92521;
  • 7 Department of Biology, Wake Forest University, Winston-Salem, NC 27109; Center for Molecular Communication and Signaling, Wake Forest University, Winston-Salem, NC 27109;
  • 8 Institute of Experimental Botany, Academy of Sciences, 165 02 Prague 6, Czech Republic; Department of Experimental Plant Biology, Faculty of Sciences, Charles University, 128 43 Prague 2, Czech Republic.
  • 9 Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521; natasha.raikhel@ucr.edu.
PMID: 26607451 DOI: 10.1073/pnas.1521248112
Abstract

The exocyst complex regulates the last steps of exocytosis, which is essential to organisms across kingdoms. In humans, its dysfunction is correlated with several significant diseases, such as diabetes and Cancer progression. Investigation of the dynamic regulation of the evolutionarily conserved exocyst-related processes using mutants in genetically tractable organisms such as Arabidopsis thaliana is limited by the lethality or the severity of phenotypes. We discovered that the small molecule Endosidin2 (ES2) binds to the EXO70 (exocyst component of 70 kDa) subunit of the exocyst complex, resulting in inhibition of exocytosis and endosomal recycling in both plant and human cells and enhancement of plant vacuolar trafficking. An EXO70 protein with a C-terminal truncation results in dominant ES2 resistance, uncovering possible distinct regulatory roles for the N terminus of the protein. This study not only provides a valuable tool in studying exocytosis regulation but also offers a potentially new target for drugs aimed at addressing human disease.

Keywords

EXO70; endosidin2; exocyst; exocytosis.

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