2. Academic Validation
  3. The Insect Peptide CopA3 Increases Colonic Epithelial Cell Proliferation and Mucosal Barrier Function to Prevent Inflammatory Responses in the Gut

The Insect Peptide CopA3 Increases Colonic Epithelial Cell Proliferation and Mucosal Barrier Function to Prevent Inflammatory Responses in the Gut

  • J Biol Chem. 2016 Feb 12;291(7):3209-23. doi: 10.1074/jbc.M115.682856.
Dae Hong Kim 1,  Jae Sam Hwang 2,  Ik Hwan Lee 1,  Seung Taek Nam 1,  Ji Hong 1,  Peng Zhang 1,  Li Fang Lu 1,  Junguee Lee 3,  Heon Seok 4,  Charalabos Pothoulakis 5,  John Thomas Lamont 6,  Ho Kim 7
Affiliations

Affiliations

  • 1 From the Department of Life Science, College of Natural Science, Daejin University, Pocheon, Gyeonggido, 487-711, Republic of Korea.
  • 2 the Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 55365, Republic of Korea.
  • 3 the Department of Pathology, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daeheung-ro 64, Jung-gu, Daejeon 301-723, Republic of Korea.
  • 4 the Department of Biomedical Engineering, Jungwon University, Goesan, Chungcheongbukdo, 367-700, South Korea.
  • 5 the Division of Digestive Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095, and.
  • 6 the Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115.
  • 7 From the Department of Life Science, College of Natural Science, Daejin University, Pocheon, Gyeonggido, 487-711, Republic of Korea, hokim@daejin.ac.kr.
PMID: 26655716 DOI: 10.1074/jbc.M115.682856
Abstract

The epithelial cells of the gut form a physical barrier against the luminal contents. The collapse of this barrier causes inflammation, and its therapeutic restoration can protect the gut against inflammation. EGF enhances mucosal barrier function and increases colonocyte proliferation, thereby ameliorating inflammatory responses in the gut. Based on our previous finding that the insect peptide CopA3 promotes neuronal growth, we herein tested whether CopA3 could increase the cell proliferation of colonocytes, enhance mucosal barrier function, and ameliorate gut inflammation. Our results revealed that CopA3 significantly increased epithelial cell proliferation in mouse colonic crypts and also enhanced colonic epithelial barrier function. Moreover, CopA3 treatment ameliorated Clostridium difficile toxin As-induced inflammation responses in the mouse small intestine (acute enteritis) and completely blocked inflammatory responses and subsequent lethality in the dextran sulfate sodium-induced mouse model of chronic colitis. The marked CopA3-induced increase of colonocyte proliferation was found to require rapid protein degradation of p21(Cip1/Waf1), and an in vitro ubiquitination assay revealed that CopA3 directly facilitated ubiquitin Ligase activity against p21(Cip1/Waf1). Taken together, our findings indicate that the insect peptide CopA3 prevents gut inflammation by increasing epithelial cell proliferation and mucosal barrier function.

Keywords

bacterial toxin; cell cycle; cell proliferation; epidermal growth factor (EGF); epithelial cell; inflammation; peptides; proteasome; protein degradation; ubiquitylation (ubiquitination).

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