2. Academic Validation
  3. Protective effects of kaempferol against reactive oxygen species-induced hemolysis and its antiproliferative activity on human cancer cells

Protective effects of kaempferol against reactive oxygen species-induced hemolysis and its antiproliferative activity on human cancer cells

  • Eur J Med Chem. 2016 May 23:114:24-32. doi: 10.1016/j.ejmech.2016.02.045.
Wenzhen Liao 1,  Luying Chen 2,  Xiang Ma 3,  Rui Jiao 4,  Xiaofeng Li 4,  Yong Wang 5
Affiliations

Affiliations

  • 1 Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
  • 2 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
  • 3 Research School of Chemistry, The Australian National University, Canberra ACT 2601, Australia.
  • 4 Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China.
  • 5 Department of Food Science and Engineering, Jinan University, 601 West, Huangpu Road, Guangzhou, 510632, China. Electronic address: twyong@jnu.edu.cn.
PMID: 26974372 DOI: 10.1016/j.ejmech.2016.02.045
Abstract

The protective effects of kaempferol against Reactive Oxygen Species (ROS)-induced hemolysis and its antiproliferative activity on human Cancer cells were evaluated in this study. Kaempferol exhibited strong cellular antioxidant ability (CAA) with a CAA value of 59.80 ± 0.379 μM of quercetin (QE)/100 μM (EC50 = 7.74 ± 0.049 μM). Pretreatment with kaempferol significantly attenuated the ROS-induced hemolysis of human erythrocyte (87.4% hemolysis suppressed at 100 μg/mL) and reduced the accumulation of toxic lipid peroxidation product malondialdehyde (MDA). The anti-hemolytic activity of kaempferol was mainly through scavenging excessive ROS and preserving the intrinsic antioxidant Enzymes (superoxide dismutase, SOD; catalase, CAT; and Glutathione Peroxidase, GPx) activities in normal levels. Additionally, kaempferol showed significant antiproliferative activity on a panel of human Cancer cell lines including human breast carcinoma (MCF-7) cells, human stomach carcinoma (SGC-7901) cells, human cervical carcinoma (Hela) cells and human lung carcinoma (A549) cells. Kaemperol induced Apoptosis of MCF-7 cells accompanied with nuclear condensation and mitochondria dysfunction.

Keywords

Anti-hemolytic activity; Antiproliferative activity; Apoptosis; Cellular antioxidant ability; Kaempferol; Reactive oxygen species.

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