2. Academic Validation
  3. Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells

Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells

  • Arch Pharm Res. 2016 Apr;39(4):474-480. doi: 10.1007/s12272-016-0730-z.
Unwoo Kang 1,  Jiyoung Park 1,  Ah-Reum Han 1,  Mi Hee Woo 2,  Je-Hyun Lee 3,  Sang Kook Lee 4,  Tong-Shin Chang 1,  Hyun Ae Woo 5,  Eun Kyoung Seo 6
Affiliations

Affiliations

  • 1 College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Korea.
  • 2 College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Korea.
  • 3 Department of Korean Medicine, Dongguk University, Gyeongju, 38066, Korea.
  • 4 College of Pharmacy, Seoul National University, Seoul, 08826, Korea.
  • 5 College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Korea. hawoo@ewha.ac.kr.
  • 6 College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Korea. yuny@ewha.ac.kr.
PMID: 26983826 DOI: 10.1007/s12272-016-0730-z
Abstract

A new cytoprotective compound, 1-[(4S)-3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl]-ethanone (1) was isolated from the flower buds of Tussilago farfara L. (Compositae), together with eight known compounds, 3,4-dicaffeoyl isoquinic acid (2), trans-cinnamic acid (3), 4-hydroxyacetophenone (4), 4,5-dicaffeoylquinic acid methyl ester (5), 3,5-dicaffeoylquinic acid methyl ester (6), 4-hydroxybenzoic acid (7), isoquercetrin (8), and ligucyperonol (9). Compounds 2-4 were found in this plant for the first time. The isolates 1-9, were tested for their cytoprotective activities against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Among them, 1 and 3 showed significant cytoprotective activities as determined by MTT assay and Lactate Dehydrogenase leakage, indicating their possibility as the potent cytoprotective agents. The structure of 1 was determined by spectroscopic data analysis including 1D- and 2D-NMR experiments, and its absolute configuration was elucidated by a circular dichroism.

Keywords

Compositae; Cytoprotection; Oxidative stress; Phenolic compound; Tussilago farfara.

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