1. Academic Validation
  2. Ecdysones from Zoanthus spp. with inhibitory activity against dengue virus 2

Ecdysones from Zoanthus spp. with inhibitory activity against dengue virus 2

  • Bioorg Med Chem Lett. 2016 May 1;26(9):2344-8. doi: 10.1016/j.bmcl.2016.03.029.
Yuan-Bin Cheng 1 Jin-Ching Lee 2 I-Wen Lo 3 Shu-Rong Chen 3 Hao-Chun Hu 3 Yu-Hsuan Wu 4 Yang-Chang Wu 5 Fang-Rong Chang 6
Affiliations

Affiliations

  • 1 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
  • 2 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 3 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
  • 4 Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • 5 School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan. Electronic address: yachwu@mail.cmu.edu.tw.
  • 6 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. Electronic address: aaronfrc@kmu.edu.tw.
Abstract

Bioassay-guided fractionation of an ethanolic extract of Zoanthus spp. collected in Taiwan has resulted in the isolation of one new ecdysone, zoanthone A (1), along with thirteen known compounds (2-14). The structures of these compounds were determined by spectroscopic methods, especially 2D NMR analyses. The in vitro Antiviral activities of all isolated ecdysones (1-14) against Dengue virus type 2 (DENV-2) were evaluated using DENV infectious system. New compound (1) exhibited potent Antiviral activity (EC50=19.61 ± 2.46 μM) with a selectivity index (CC50/EC50) value of 36.7. The structure-activity relationships of isolated ecdysones against DENV-2 were concluded. Molecular docking information of 3 and NS5 polymerase was performed either.

Keywords

Dengue virus type 2; Ecdysone; Molecular docking; Structure–activity relationships; Zoanthus.

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