2. Academic Validation
  3. New Insights into the Antibacterial Activity of Hydroxycoumarins against Ralstonia solanacearum

New Insights into the Antibacterial Activity of Hydroxycoumarins against Ralstonia solanacearum

  • Molecules. 2016 Apr 8;21(4):468. doi: 10.3390/molecules21040468.
Liang Yang 1,  Wei Ding 2,  Yuquan Xu 3,  Dousheng Wu 4,  Shili Li 5,  Juanni Chen 6,  Bing Guo 7
Affiliations

Affiliations

  • 1 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. ylwzling@163.com.
  • 2 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. dwing818@163.com.
  • 3 Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences, Beijing 100081, China. xuyuquan@caas.cn.
  • 4 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. swudousheng@163.com.
  • 5 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. lsl203lst@163.com.
  • 6 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. chenhuanni521@126.com.
  • 7 Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. guobing425@163.com.
PMID: 27070570 DOI: 10.3390/molecules21040468
Abstract

Coumarins are important plant-derived Natural Products with wide-ranging bioactivities and extensive applications. In this study, we evaluated for the first time the Antibacterial activity and mechanisms of action of Coumarins against the phytopathogen Ralstonia solanacearum, and investigated the effect of functional group substitution. We first tested the Antibacterial activity of 18 plant-derived Coumarins with different substitution patterns, and found that daphnetin, esculetin, xanthotol, and umbelliferone significantly inhibited the growth of R. solanacearum. Daphnetin showed the strongest Antibacterial activity, followed by esculetin and umbelliferone, with MICs of 64, 192, and 256 mg/L, respectively, better than the archetypal coumarin with 384 mg/L. We further demonstrated that the hydroxylation of Coumarins at the C-6, C-7 or C-8 position significantly enhanced the Antibacterial activity against R. solanacearum. Transmission electron microscope (TEM) and fluorescence microscopy images showed that hydroxycoumarins may interact with the pathogen by mechanically destroying the cell membrane and inhibiting biofilm formation. The antibiofilm effect of hydroxycoumarins may relate to the repression of flagellar genes fliA and flhC. These physiological changes in R. solanacearum caused by hydroxycoumarins can provide information for integral pathogen control. The present findings demonstrated that hydroxycoumarins have superior Antibacterial activity against the phytopathogen R. solanacearum, and thus have the potential to be applied for controlling plant Bacterial wilt.

Keywords

Ralstonia solanacearum; antibacterial activity; bacterial wilt; biofilm inhibition; daphnetin; esculetin; hydroxycoumarins.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z