2. Academic Validation
  3. Fluorogenic "photoclick" labelling of DNA using a Cy3 dye

Fluorogenic "photoclick" labelling of DNA using a Cy3 dye

  • Org Biomol Chem. 2018 Nov 7;16(41):7579-7582. doi: 10.1039/c8ob02068j.
Benjamin Lehmann 1,  Hans-Achim Wagenknecht 1
Affiliations

Affiliation

  • 1 Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany. Wagenknecht@kit.edu.
PMID: 30307458 DOI: 10.1039/c8ob02068j
Abstract

Two 2'-deoxyuridines as new building blocks for automated DNA synthesis carry a small aryltetrazole as "photoclickable" group at their 5-positions. The postsynthetic "photoclick" labeling of such presynthesized DNA using a maleimide-modified Cy3 dye shows an up to 17-fold fluorogenicity due to an energy transfer between the pyrazoline moiety and the Cy3 fluorophore in the DNA products. This concept is also applicable to Other maleimide-modified dyes.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z