2. Academic Validation
  3. Anti-Inflammatory Activities of Compounds Isolated from the Rhizome of Anemarrhena asphodeloides

Anti-Inflammatory Activities of Compounds Isolated from the Rhizome of Anemarrhena asphodeloides

  • Molecules. 2018 Oct 13;23(10):2631. doi: 10.3390/molecules23102631.
Zeyuan Wang 1,  Jianfeng Cai 2,  Qing Fu 3,  Lingping Cheng 4,  Lehao Wu 5,  Weiyue Zhang 6,  Yan Zhang 7,  Yu Jin 8,  Chunzhi Zhang 9
Affiliations

Affiliations

  • 1 School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China. wzydevil@126.com.
  • 2 Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. 921377549@163.com.
  • 3 Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. fuqing@ecust.edu.cn.
  • 4 Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. clp0708@163.com.
  • 5 School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. wulehaogo@sjtu.edu.cn.
  • 6 School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. zhangweiyue@sjtu.edu.cn.
  • 7 School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. zhangyan_sjtu@sjtu.edu.cn.
  • 8 Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. jiny@ecust.edu.cn.
  • 9 School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China. zhangcz@dlpu.edu.cn.
PMID: 30322157 DOI: 10.3390/molecules23102631
Abstract

Fifteen unreported compounds in Anemarrhena asphodeloides, iriflophene (3), hostaplantagineoside C (7), tuberoside G (8), spicatoside B (9), platycodin D (14), platycoside A (15), platycodin D2 (16), polygalacin D2 (17), platycodin D3 (18), isovitexin (20), vitexin (21), 3,4-dihydroxyallylbenzene-3-O-α-l-rhamnopyranosyl(1→6)-β-d-glucopyranoside (22), iryptophan (24), adenosine (25), α-d-Glucose monoallyl ether (26), together with eleven known compounds (1, 2, 4⁻6, 10⁻13, 19 and 23), were isolated from the rhizomes of Anemarrhena asphodeloides. The chemical structures of these compounds were characterized using HRMS and NMR. The anti-inflammatory activities of the compounds were evaluated by investigating their ability to inhibit LPS-induced NO production in N9 microglial cells. Timosaponin BIII (TBIII) and trans-hinokiresinol (t-HL) exhibited significant inhibitory effects on the NO production in a dose-dependent manner with IC50 values of 11.91 and 39.08 μM, respectively. Immunoblotting demonstrated that TBIII and t-HL suppressed NO production by inhibiting the expressions of iNOS in LPS-stimulated N9 microglial cells. Further results revealed that pretreatment of N9 microglial cells with TBIII and t-HL attenuated the LPS-induced expression tumor necrosis factor (TNF)-α and interleukin-6 (IL-6) at mRNAs and protein levels. Moreover, the activation of nuclear factor-κB (NF-κB) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways were inhibited by TBIII and t-HL, respectively. Our findings indicate that the therapeutic implication of TBIII and t-HL for neurogenerative disease associated with neuroinflammation.

Keywords

Anemarrhena asphodeloides; N9; anti-inflammation; purification.

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