1. Academic Validation
  2. Synthetic Semiflexible and Bioactive Brushes

Synthetic Semiflexible and Bioactive Brushes

  • Biomacromolecules. 2019 Jul 8;20(7):2587-2597. doi: 10.1021/acs.biomac.9b00385.
Dion Voerman Marjolein Schluck Jorieke Weiden Ben Joosten Loek J Eggermont Tuur van den Eijnde 1 Bob Ignacio 1 Alessandra Cambi Carl G Figdor Paul H J Kouwer 1 Martijn Verdoes Roel Hammink Alan E Rowan 1 2
Affiliations

Affiliations

  • 1 Department of Molecular Materials , Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135 , 6525 AJ Nijmegen , The Netherlands.
  • 2 Australian Institute for Bioengineering and Nanotechnology , The University of Queensland , Brisbane , QLD 4072 , Australia.
Abstract

Polymer brushes are extensively used for the preparation of bioactive surfaces. They form a platform to attach functional (bio)molecules and control the physicochemical properties of the surface. These brushes are nearly exclusively prepared from flexible Polymers, even though much stiffer brushes from semiflexible Polymers are frequently found in nature, which exert bioactive functions that are out of reach for flexible brushes. Synthetic semiflexible Polymers, however, are very rare. Here, we use polyisocyanopeptides (PICs) to prepare high-density semiflexible brushes on different substrate geometries. For bioconjugation, we developed routes with two orthogonal click reactions, based on the strain-promoted azide-alkyne cycloaddition reaction and the (photoactivated) tetrazole-ene cycloaddition reaction. We found that for high brush densities, multiple bonds between the polymer and the substrate are necessary, which was achieved in a block copolymer strategy. Whether the desired biomolecules are conjugated to the PIC polymer before or after brush formation depends on the dimensions and required densities of the biomolecules and the curvature of the substrate. In either case, we provide mild, aqueous, and highly modular reaction strategies, which make PICs a versatile addition to the toolbox for generating semiflexible bioactive polymer brush surfaces.

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