2. Academic Validation
  3. Investigating the effect of radiosensitizer for Ursolic Acid and Kamolonol Acetate ‌ on HCT-116 cell line

Investigating the effect of radiosensitizer for Ursolic Acid and Kamolonol Acetate ‌ on HCT-116 cell line

  • Bioorg Med Chem. 2020 Jan 1;28(1):115152. doi: 10.1016/j.bmc.2019.115152.
Maede Hasan Abdali 1,  Saeid Afshar 2,  Abdolazim Sedighi Pashaki 3,  Dara Dastan 4,  Mohammad Hadi Gholami 3,  Roghayeh Mahmoudi 2,  Massoud Saidijam 5
Affiliations

Affiliations

  • 1 Department of Medical Physics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
  • 2 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
  • 3 Mahdieh Radiotherapy and Brachytherapy Charitable Center, Hamadan, Iran.
  • 4 Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
  • 5 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: sjam110@yahoo.com.
PMID: 31771799 DOI: 10.1016/j.bmc.2019.115152
Abstract

Purpose: The aim of this study was evaluating the cytotoxic and radiosensitizing effects of Ursolic Acid (UA) and Kamolonol Acetate (KA) on HCT116 cell line and finally investigating the functional role of NF-κB and CCND1 genes in the radiosensitizing activity of UA and KA.

Materials and method: The cytotoxic effects of UA and KA by MTT assay was evaluated on HCT-116. Clonogenic assay was performed to investigate of radiosensitizing effects of UA and KA on HCT116. To assessment the expression levels of NF-κB and CCND1 genes, Real-Time PCR method was used.

Results: The results of MTT assay revealed that UA and KA have cytotoxic effects on HCT116 cell line. According to clonogenic assay, survival fraction of treated cells with UA and KA has been decreased compared to the survival fraction of untreated cells. UA and KA lead to the decrease in the expression level of NF-κB. Synergistic effect of radiosensitizing agents with radiation was only approved for UA and 2 Gy of radiation.

Conclusion: Based on our study, UA and KA have cytotoxic effects on HCT116 cell line. Furthermore, UA may lead to radiosensitization of human colorectal tumor cells by NF-κB1 and CCND1signaling pathways.

Keywords

HCT116 cells; Kamolonol Acetate; Radiobiology; Radiotherapy; Ursolic Acid.

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