2. Academic Validation
  3. Small ultra-red fluorescent protein nanoparticles as exogenous probes for noninvasive tumor imaging in vivo

Small ultra-red fluorescent protein nanoparticles as exogenous probes for noninvasive tumor imaging in vivo

  • Int J Biol Macromol. 2020 Jun 15;153:100-106. doi: 10.1016/j.ijbiomac.2020.02.253.
Feifei An 1,  Nandi Chen 2,  William J Conlon 3,  Justin S Hachey 3,  Jingqi Xin 4,  Omer Aras 5,  Erik A Rodriguez 6,  Richard Ting 7
Affiliations

Affiliations

  • 1 Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Science, Health Science Center, Xi'an Jiaotong University, No.76 Yanta West Road, Xi'an, Shaanxi 710061, PR China.; Department of Radiology, Weill Cornell Medicine, New York, NY 10065, USA.
  • 2 Department of Radiology, Weill Cornell Medicine, New York, NY 10065, USA.; Department of Gastrointestinal Surgery, The Second Clinical Medicine College (Shenzhen People's Hospital) of Jinan University, Shenzhen, Guangdong 518020, China.
  • 3 Department of Chemistry, The George Washington University, Washington, DC 20052, USA.
  • 4 Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Science, Health Science Center, Xi'an Jiaotong University, No.76 Yanta West Road, Xi'an, Shaanxi 710061, PR China.
  • 5 Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
  • 6 Department of Chemistry, The George Washington University, Washington, DC 20052, USA.. Electronic address: erik_rodriguez@gwu.edu.
  • 7 Department of Radiology, Weill Cornell Medicine, New York, NY 10065, USA.. Electronic address: rct2001@med.cornell.edu.
PMID: 32105698 DOI: 10.1016/j.ijbiomac.2020.02.253
Abstract

Nanoparticles are excellent imaging agents for Cancer, but variability in chemical structure, racemic mixtures, and addition of heavy metals hinders FDA approval in the United States. We developed a small ultra-red fluorescent protein, named smURFP, to have optical properties similar to the small-molecule Cy5, a heptamethine subclass of cyanine dyes (Ex/Em = 642/670 nm). smURFP has a fluorescence quantum yield of 18% and expresses so well in E. coli, that gram quantities of fluorescent protein are purified from cultures in the laboratory. In this research, the fluorescent protein smURFP was combined with bovine serum albumin into fluorescent protein nanoparticles. These nanoparticles are fluorescent with a quantum yield of 17% and 12-14 nm in diameter. The far-red fluorescent protein nanoparticles noninvasively image tumors in living mice via the enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticle for in vivo fluorescent imaging. This protein nanoparticle core should prove useful as a biomacromolecular scaffold, which could bear extended chemical modifications for studies, such as the in vivo imaging of fluorescent protein nanoparticles targeted to primary and metastatic Cancer, theranostic treatment, and/or dual-modality imaging with positron emission tomography for entire human imaging.

Keywords

Cancer imaging; Far-red fluorescence; Fluorescent protein; Protein nanoparticle; Tumor imaging.

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