2. Academic Validation
  3. Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy

Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy

  • Cell Death Dis. 2020 Apr 27;11(4):288. doi: 10.1038/s41419-020-2473-5.
Hong-Xu Chen 1 2,  Fu-Chao Liang 2 3,  Ping Gu 1,  Bian-Ling Xu 2 4,  Hong-Jun Xu 2 3,  Wen-Ting Wang 1,  Jia-Yang Hou 2 3,  Dong-Xiao Xie 5,  Xi-Qing Chai 6,  Sheng-Jun An 7 8
Affiliations

Affiliations

  • 1 Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China.
  • 2 Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China.
  • 3 Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China.
  • 4 Department of Gynecology, the Second Hospital of Hebei Medical University, No. 215, HePing West Road, Shi Jiazhuang, 050000, Hebei, China.
  • 5 Department of orthopaedic, Third hospital of Hebei Medical University, Shi Jiazhuang, 050000, Hebei, China.
  • 6 Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China. chenhbykdx@126.com.
  • 7 Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. sjsjan@126.com.
  • 8 Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. sjsjan@126.com.
PMID: 32341347 DOI: 10.1038/s41419-020-2473-5
Abstract

Parkinson's disease (PD) is a progressively debilitating neurodegenerative condition that leads to motor and cognitive dysfunction. At present, clinical treatment can only improve symptoms, but cannot effectively protect dopaminergic neurons. Several reports have demonstrated that human umbilical cord mesenchymal stem cells (hucMSCs) afford neuroprotection, while their application is limited because of their uncontrollable differentiation and other reasons. Stem cells communicate with cells through secreted exosomes (Exos), the present study aimed to explore whether Exos secreted by hucMSCs could function instead of hucMSCs. hucMSCs were successfully isolated and characterized, and shown to contribute to 6-hydroxydopamine (6-OHDA)-stimulated SH-SY5Y cell proliferation; hucMSC-derived Exos were also involved in this process. The Exos were purified and identified, and then labeled with PKH 26, it was found that the Exos could be efficiently taken up by SH-SY5Y cells after 12 h of incubation. Pretreatment with Exos promoted 6-OHDA-stimulated SH-SY5Y cells to proliferate and inhibited Apoptosis by inducing Autophagy. Furthermore, Exos reached the substantia nigra through the blood-brain barrier (BBB) in vivo, relieved apomorphine-induced asymmetric rotation, reduced substantia nigra dopaminergic neuron loss and Apoptosis, and upregulated the level of dopamine in the striatum. These results demonstrate that hucMSCs-Exos have a treatment capability for PD and can traverse the BBB, indicating their potential for the effective treatment of PD.

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