2. Academic Validation
  3. Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

  • Nanoscale. 2020 Oct 15;12(39):20287-20291. doi: 10.1039/d0nr03694c.
Christian Heck 1,  Dmitry Torchinsky 1,  Gil Nifker 1,  Felix Gularek 2,  Yael Michaeli 1,  Elmar Weinhold 2,  Yuval Ebenstein 1
Affiliations

Affiliations

  • 1 School of Chemistry/Center for Nanoscience and Nanotechnology/Center for Light-Matter Interaction, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel. check@tauex.tau.ac.il uv@post.tau.ac.il.
  • 2 Institute of Organic Chemistry, RWTH Aachen University, D-52056 Aachen, Germany.
PMID: 33001091 DOI: 10.1039/d0nr03694c
Abstract

Non-DNA labels are key components for the construction of functional DNA nanostructures. Here, we present a method to graft covalent labels onto DNA origami nanostructures in an enzymatic one-pot reaction. The DNA Methyltransferase M.TaqI labels the DNA nanostructures with Azide groups, which serve as universal attachment points via Click Chemistry. Direct labeling with fluorescent dyes is also demonstrated. The procedure yields structures with high fluorescence intensities and narrow intensity distributions. In combination with UV crosslinking it enables the creation of temperature-stable, intense fluorescent beacons.

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