1. Academic Validation
  2. Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

  • Oncol Rep. 2020 Dec;44(6):2465-2474. doi: 10.3892/or.2020.7813.
Xiliang Guan  # 1 Yufen Xu  # 2 Jufen Zheng 3
Affiliations

Affiliations

  • 1 Department of Orthopaedic Surgery, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China.
  • 2 Department of Oncology, The First Hospital of Jiaxing and The Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China.
  • 3 The Department of Bone, Zhejiang Hospital, Hangzhou, Zhejiang 310030, P.R. China.
  • # Contributed equally.
Abstract

Osteosarcoma is a severe malignant tumor. Several studies indicated that lncRNA prostate cancer‑associated transcript 6 (PCAT6) promoted the development of multiple types of cancers. Studies have also revealed that MDM2 could aggravate tumor symptoms inhibiting P53 expression. However, whether lncRNA PCAT6 could affect the proliferation and metastasis of osteosarcoma cells by regulating P53 expression is unclear. The present study established lncRNA PCAT6‑overexpressing osteosarcoma cells. Cell Counting Kit‑8, wound healing and Transwell assays were performed to detect the change in proliferation, migration and invasion of these cells, respectively. Subsequently, E3 ubiquitin‑protein Ligase Mdm2 (MDM2), P53 and P21 expression were determined using western blotting. Finally, MDM2 expression was inhibited and the proliferation, migration and invasion of these cells was determined again. The present study found that the proliferation, migration and invasion of osteosarcoma cells increased following overexpression of lncRNA PCAT6. MDM2 expression was upregulated while the levels of P53 and P21 decreased following overexpression of lncRNA PCAT6. However, the proliferation, migration and invasion of osteosarcoma cells were inhibited following MDM2 knockdown. Additionally, P53 and P21 was rescued following MDM2 knockdown. To conclude, lncRNA PCAT6 promoted the proliferation, migration and invasion of osteosarcoma cells by promoting the expression of MDM2 and suppressing the expression of P53 and P21.

Keywords

osteosarcoma; long non-coding RNA prostate cancer-associated transcript 6; E3 ubiquitin-protein ligase Mdm2; P53; proliferation.

Figures
Products