2. Academic Validation
  3. Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome

Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome

  • Cell Tissue Res. 2021 Mar;383(3):1167-1182. doi: 10.1007/s00441-020-03341-3.
Zhen-Yu She  # 1 2,  Kai-Wei Yu  # 3 4,  Ya-Lan Wei  # 5 6,  Ning Zhong 3 4,  Yang Lin 3 4
Affiliations

Affiliations

  • 1 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, Fujian, China. zhenyushe@fjmu.edu.cn.
  • 2 Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, 350122, Fujian, China. zhenyushe@fjmu.edu.cn.
  • 3 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, Fujian, China.
  • 4 Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, 350122, Fujian, China.
  • 5 Fujian Obstetrics and Gynecology Hospital, Fuzhou, 350011, Fujian, China.
  • 6 Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, China.
  • # Contributed equally.
PMID: 33237480 DOI: 10.1007/s00441-020-03341-3
Abstract

The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential Kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the formation of the acrosome during spermatogenesis. Male ICR mice are injected with GSK923295 for long-term inhibition of CENP-E. Chemical inhibition and siRNA-mediated knockdown of CENP-E are carried out in the GC-2 spd cells. The morphology of the acrosomes is determined by the HE staining, immunofluorescence, and transmission electron microscopy. We have identified CENP-E is a key factor in the formation and structural maintenance of the acrosome during acrosome biogenesis. Long-term inhibition of CENP-E by GSK923295 results in the asymmetric acrosome and the dispersed acrosome. CENP-E depletion leads to the malformation of the Golgi complex and abnormal targeting of the PICK1- and PIST-positive Golgi-associated vesicles. Our findings uncover an essential role of CENP-E in membrane trafficking and structural organization of the acrosome in the spermatids during spermatogenesis. Our results shed LIGHT on the molecular mechanisms involved in vesicle trafficking and architecture maintenance of the acrosome.

Keywords

Acrosome; CENP-E; Kinesin-7; Spermatogenesis; The Golgi complex.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z