2. Academic Validation
  3. m6AmPred: Identifying RNA N6, 2'-O-dimethyladenosine (m6Am) sites based on sequence-derived information

m6AmPred: Identifying RNA N6, 2'-O-dimethyladenosine (m6Am) sites based on sequence-derived information

  • Methods. 2022 Jul;203:328-334. doi: 10.1016/j.ymeth.2021.01.007.
Jie Jiang 1,  Bowen Song 2,  Kunqi Chen 3,  Zhiliang Lu 4,  Rong Rong 4,  Yu Zhong 4,  Jia Meng 5
Affiliations

Affiliations

  • 1 Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, L7 8TX Liverpool, United Kingdom.
  • 2 Department of Mathematical Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, L7 8TX Liverpool, United Kingdom. Electronic address: Bowen.Song@liverpool.ac.uk.
  • 3 Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China; Institute of Ageing & Chronic Disease, University of Liverpool, L7 8TX Liverpool, United Kingdom.
  • 4 Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China.
  • 5 Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China; AI University Research Centre, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, L7 8TX Liverpool, United Kingdom.
PMID: 33540081 DOI: 10.1016/j.ymeth.2021.01.007
Abstract

N6,2'-O-dimethyladenosine (m6Am) is a reversible modification widely occurred on varied RNA molecules. The biological function of m6Am is yet to be known though recent studies have revealed its influences in cellular mRNA fate. Precise identification of m6Am sites on RNA is vital for the understanding of its biological functions. We present here m6AmPred, the first web server for in silico identification of m6Am sites from the primary sequences of RNA. Built upon the eXtreme Gradient Boosting with Dart algorithm (XgbDart) and EIIP-PseEIIP encoding scheme, m6AmPred achieved promising prediction performance with the AUCs greater than 0.954 when tested by 10-fold cross-validation and independent testing datasets. To critically test and validate the performance of m6AmPred, the experimentally verified m6Am sites from two data sources were cross-validated. The m6AmPred web server is freely accessible at: https://www.xjtlu.edu.cn/biologicalsciences/m6am, and it should make a useful tool for the researchers who are interested in N6,2'-O-dimethyladenosine RNA modification.

Keywords

EIIP-PseEIIP; Feature analysis; N6,2′-O-dimethyladenosine (m(6)A(m)); Sequence-derived features; eXtreme Gradient Boosting (XgbDart).

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z