1. Academic Validation
  2. Exogenous L-carnitine ameliorates burn-induced cellular and mitochondrial injury of hepatocytes by restoring CPT1 activity

Exogenous L-carnitine ameliorates burn-induced cellular and mitochondrial injury of hepatocytes by restoring CPT1 activity

  • Nutr Metab (Lond). 2021 Jun 24;18(1):65. doi: 10.1186/s12986-021-00592-x.
Pengtao Li  # 1 2 Zhengguo Xia  # 3 Weichang Kong 1 Qiong Wang 2 Ziyue Zhao 2 Ashley Arnold 4 Qinglian Xu 5 Jiegou Xu 6
Affiliations

Affiliations

  • 1 Department of Burns, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China.
  • 2 Department of Immunology, School of Basic Medical Sciences of Anhui Medical University, No. 81 Meishan Road, Hefei, 230032, Anhui, China.
  • 3 Department of Wound Repair and Plastic and Aesthetic Surgery, The Fourth Affiliated Hospital of Anhui Medical University, No. 100 Huaihai Road, Xinzhan District, China.
  • 4 International College of Anhui Medical University, No. 81 Meishan road, Hefei, 230032, Anhui, China.
  • 5 Department of Burns, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China. xuqinglian@sina.com.
  • 6 Department of Immunology, School of Basic Medical Sciences of Anhui Medical University, No. 81 Meishan Road, Hefei, 230032, Anhui, China. xujiegou@ahmu.edu.cn.
  • # Contributed equally.
Abstract

Background: Impaired hepatic fatty acid metabolism and persistent mitochondrial dysfunction are phenomena commonly associated with liver failure. Decreased serum levels of L-carnitine, a amino acid derivative involved in fatty-acid and energy metabolism, have been reported in severe burn patients. The current study aimed to evaluate the effects of L-carnitine supplementation on mitochondrial damage and other hepatocyte injuries following severe burns and the related mechanisms.

Methods: Serum carnitine and other Indicators of hepatocytic injury, including AST, ALT, LDH, TG, and OCT, were analyzed in severe burn patients and healthy controls. A burn model was established on the back skin of rats; thereafter, carnitine was administered, and serum levels of the above Indicators were evaluated along with Oil Red O and TUNEL staining, transmission electron microscopy, and assessment of mitochondrial membrane potential and carnitine palmitoyltransferase 1 (CPT1) activity and expression levels in the liver. HepG2 cells pretreated with the CPT1 inhibitor etomoxir were treated with or without carnitine for 24 h. Next, the above Indicators were examined, and apoptotic cells were analyzed via flow cytometry. High-throughput Sequencing of rat liver tissues identified several differentially expressed genes (Fabp4, Acacb, Acsm5, and Pnpla3) were confirmed using RT-qPCR.

Results: Substantially decreased serum levels of carnitine and increased levels of AST, ALT, LDH, and OCT were detected in severe burn patients and the burn model rats. Accumulation of TG, evident mitochondrial shrinkage, altered mitochondrial membrane potential, decreased ketogenesis, and reduced CPT1 activity were detected in the liver tissue of the burned rats. Carnitine administration recovered CPT1 activity and improved all Indicators related to cellular and fatty acid metabolism and mitochondrial injury. Inhibition of CPT1 activity with etomoxir induced hepatocyte injuries similar to those in burn patients and burned rats; carnitine supplementation restored CPT1 activity and ameliorated these injuries. The expression levels of the differentially expressed genes Fabp4, Acacb, Acsm5, and Pnpla3 in the liver tissue from burned rats and etomoxir-treated hepatocytes were also restored by treatment with exogenous carnitine.

Conclusion: Exogenous carnitine exerts protective effects against severe burn-induced cellular, fatty-acid metabolism, and mitochondrial dysfunction of hepatocytes by restoring CPT1 activity.

Keywords

Burn; CPT1; Carnitine; Mitochondrial injury.

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