2. Academic Validation
  3. Ex vivo to in vivo extrapolation of syringic acid and ferulic acid as grape juice proxies for endothelium-dependent vasodilation: Redefining vasoprotective resveratrol of the French paradox

Ex vivo to in vivo extrapolation of syringic acid and ferulic acid as grape juice proxies for endothelium-dependent vasodilation: Redefining vasoprotective resveratrol of the French paradox

  • Food Chem. 2021 Nov 30;363:130323. doi: 10.1016/j.foodchem.2021.130323.
Yunke Huang 1,  Min Xu 2,  Junfeng Li 2,  Ken Chen 2,  Liang Xia 2,  Wei Wang 2,  Ping Ren 3,  Xi Huang 4
Affiliations

Affiliations

  • 1 Institute of TCM-Related Comorbid Depression, Nanjing University of Chinese Medicine, Nanjing 210023, China; Women's Hospital School of Medicine Zhejiang University, Hangzhou 310006, China.
  • 2 Institute of TCM-Related Comorbid Depression, Nanjing University of Chinese Medicine, Nanjing 210023, China.
  • 3 Institute of TCM-Related Comorbid Depression, Nanjing University of Chinese Medicine, Nanjing 210023, China; Affiliated Hospital Nanjing University of Chinses Medicine, Nanjing 210029, China.
  • 4 Institute of TCM-Related Comorbid Depression, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: tcmhuangx59@163.com.
PMID: 34247035 DOI: 10.1016/j.foodchem.2021.130323
Abstract

Resveratrol, in wines, has been implicated to be primarily responsible for the French paradox, remaining controversial. Herein, we elucidated the representative vasodilation-increasing Polyphenols from concord grape juice (CGJ) using ex vivo-to-in vivo extrapolation (EVIVE). We verified the interference-free antioxidant response of CGJ post-dose supernatant of deproteinated serum (CPSDS, as ex vivo proxy) in isolated aortic rings, and in healthy, and H2O2-treated endothelial cells (H-ECs). Syringic acid and ferulic acid (SF) were detected in CGJ and post-dose rat serum (PRS). In isolated aortic rings and H-ECs, Polyphenols alone, or in combination, at doses equivalent to those detected in PRS, quantitatively reflected endothelium-dependent vasodilation of CPSDS, as evidenced by nitric oxide (NO) formation-mediated antioxidation-sensitive activation of Src kinase with subsequent PI3/Akt-dependent phosphorylation of endothelial NO Synthase. Using EVIVE, SF closely reflected CGJ in coronary flow-mediated vasodilation. Hence, SF application in precision ethnomedicine may redefine antioxidant-sensitive vasoprotective resveratrol of the French paradox.

Keywords

Ferulic acid; French paradox; Grape juice; Resveratrol; Syringic acid; Vasodilate.

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