2. Academic Validation
  3. Visualization of lipophagy using a supramolecular FRET pair

Visualization of lipophagy using a supramolecular FRET pair

  • Chem Commun (Camb). 2021 Nov 16;57(91):12179-12182. doi: 10.1039/d1cc04779e.
Ara Lee 1 2,  Meng Li 1,  Young Ho Ko 1,  Sanghwang Park 3,  Jongcheol Seo 3,  Kyeng Min Park 4,  Kimoon Kim 1 2 3
Affiliations

Affiliations

  • 1 Center for Self-assembly and Complexity (CSC), Institute for Basic Science (IBS), Pohang 37673, Republic of Korea. kkim@postech.ac.kr.
  • 2 Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
  • 3 Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
  • 4 Department of Biochemistry, Daegu Catholic University School of Medicine, Daegu 42471, Republic of Korea. kpark@cu.ac.kr.
PMID: 34730134 DOI: 10.1039/d1cc04779e
Abstract

A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbit[7]uril (Cy3-CB[7]) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific Autophagy events. The intracellular accumulations of Cy3-CB[7] in lysosomes and BDP-AdA in lipid droplets (LDs) and the formation of an intracellular host-guest complex between Cy3-CB[7] and BDP-AdA resulting in FRET signals allow us to visualize the fusion of LDs with lysosomes, namely, lipophagy. This study demonstrates the potential of supramolecular imaging based on bio-orthogonal host-guest interactions in the investigation of selective Autophagy events.

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