2. Academic Validation
  3. MrgprX2 regulates mast cell degranulation through PI3K/AKT and PLCγ signaling in pseudo-allergic reactions

MrgprX2 regulates mast cell degranulation through PI3K/AKT and PLCγ signaling in pseudo-allergic reactions

  • Int Immunopharmacol. 2022 Jan;102:108389. doi: 10.1016/j.intimp.2021.108389.
Fan Zhang 1,  Fang Hong 1,  Lu Wang 2,  Renjie Fu 3,  Jin Qi 4,  Boyang Yu 5
Affiliations

Affiliations

  • 1 Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China; Research Center for Traceability and Standardization of TCMs, China Pharmaceutical University, Nanjing 211198, China.
  • 2 Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China; Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, China.
  • 3 Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China.
  • 4 Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China. Electronic address: qijin2006@163.com.
  • 5 Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China; Research Center for Traceability and Standardization of TCMs, China Pharmaceutical University, Nanjing 211198, China. Electronic address: boyangyu59@163.com.
PMID: 34920312 DOI: 10.1016/j.intimp.2021.108389
Abstract

The G protein-coupled receptor MrgprX2 in mast cells is known to be a crucial receptor for pseudo-allergic reactions. MrgprX2 activation leads to elevated intracellular calcium levels and mast cell degranulation, but the underlying mechanism remains to be elucidated. Herein, we investigated the role of the phosphatidylinositol 3 kinase (PI3K)/serum-threonine kinase (Akt) signaling pathway and Phospholipase C gamma (PLCγ) in mast cell degranulation mediated by MrgprX2 in LAD2 human-derived mast cells. The results showed that phosphorylated Akt (p-AKT) and PLCγ up-regulation were accompanied by an increase in intracellular calcium following activation of MrgprX2 by Compound 48/80, an inducer of mast cell degranulation. In contrast, p-AKT and PLCγ were down-regulated and intracellular calcium levels decreased after MrgprX2 knockdown. Mast cell degranulation was clearly suppressed; however, inhibiting PI3K and PLCγ phosphorylation did not influence MrgprX2 expression. The increase in calcium concentration was suppressed and mast cell degranulation was weakened. Furthermore, by inhibiting PI3K and PLCγ phosphorylation in Animals, the allergic symptoms caused by C48/80 were obviously reduced. We deduced that during the mast cell degranulation observed in pseudoallergic reactions, MrgprX2 regulated intracellular calcium levels via the PI3K/Akt and PLCγ pathways.

Keywords

Mast cell; MrgprX2; PI3K/AKT; PLCγ; Pseudo-allergic reactions.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z