2. Academic Validation
  3. WDR62 regulates mouse oocyte meiotic maturation related to p-JNK and H3K9 trimethylation

WDR62 regulates mouse oocyte meiotic maturation related to p-JNK and H3K9 trimethylation

  • Int J Biochem Cell Biol. 2022 Mar;144:106169. doi: 10.1016/j.biocel.2022.106169.
Yong-Sheng Wang 1,  Chao Chen 1,  Muhammad Jamil Ahmad 1,  Fan Chen 1,  Zhi-Ming Ding 1,  Sheng-Ji Yang 1,  Yang-Wu Chen 1,  Ze-Qun Duan 1,  Ming Liu 1,  Ai-Xin Liang 1,  Chang-Jiu He 1,  Guo-Hua Hua 2,  Li-Jun Huo 3
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • 2 College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: huaguohua09@gmail.com.
  • 3 College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: lijunhuo@yahoo.com.
PMID: 35093571 DOI: 10.1016/j.biocel.2022.106169
Abstract

WDR62 (WD40-repeat protein 62) participates in diverse biological process, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein. However, the role of WDR62 exerts in spindle assembly and meiotic progression control in oocytes lacking typical centrosomes remains obscure. In a previous study, we reported that WDR62 is involved in spindle migration and asymmetric cytokinesis in mouse oocyte meiosis. In the current study, another novel function of WDR62 regulating cell cycle progression through meiotic spindle formation during oocyte meiotic maturation was found. Knockdown of WDR62 through siRNA microinjection disrupted the meiotic cell cycle and induced metaphase-I (MI) arrest coupled with severe spindle abnormality, chromosome misalignment, and aneuploid generation. Moreover, WDR62 depletion induced defective kinetochore-microtubule attachments (K-MT) and activated spindle assembly checkpoint (SAC), which could trigger the arrest of meiotic progression. Further study demonstrated that depletion of WDR62 was associated with an aberrant location of p-JNK and reduced its expression level; concomitantly, status of H3K9 trimethylation was also altered. In addition, phenotypes similar to WDR62 depletion were observed during the function-loss analysis of p-JNK using a specific inhibitor (SP600125), which signifies that WDR62 is important for spindle organization and meiotic progression, and this function might be via its regulation of p-JNK. In conclusion, this study revealed that WDR62 functions in multiple ways during oocyte meiotic maturation, which could be related to p-JNK and H3K9 trimethylation.

Keywords

H3K9me3; Meiotic progression; Oocyte; P-JNK; Spindle assembly; WDR62.

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