2. Academic Validation
  3. MEIOK21 regulates oocyte quantity and quality via modulating meiotic recombination

MEIOK21 regulates oocyte quantity and quality via modulating meiotic recombination

  • FASEB J. 2022 Jun;36(6):e22357. doi: 10.1096/fj.202101950R.
Yongliang Shang 1 2,  Ju Huang 1 3 4 5,  Weidong Li 1 2,  Yanan Zhang 1 3 4 5,  Xu Zhou 1 2,  Qiqi Shao 1 2,  Taicong Tan 1,  Shen Yin 6,  Liangran Zhang 1 2 7,  Shunxin Wang 1 3 4 5
Affiliations

Affiliations

  • 1 Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.
  • 2 Advanced Medical Research Institute, Shandong University, Jinan, China.
  • 3 National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China.
  • 4 Key Laboratory of Reproductive Endocrinology of Ministry of Education, Jinan, China.
  • 5 Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China.
  • 6 College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
  • 7 Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, China.
PMID: 35593531 DOI: 10.1096/fj.202101950R
Abstract

The reproductive life span of females is largely determined by the number and quality of oocytes. Previously, we identified MEIOK21 as a meiotic recombination regulator required for male fertility. Here, we characterize the important roles of MEIOK21 in regulating female meiosis and oocyte number and quality. MEIOK21 localizes at recombination sites as a component of recombination bridges in oogenesis like in spermatogenesis. Meiok21-/- female mice show subfertility. Consistently, the size of the primordial follicle pool in Meiok21-/- females is only ~40% of wild-type females because a great number of oocytes with defects in meiotic recombination and/or synapsis are eliminated. Furthermore, the numbers of primordial and growing follicles show a more marked decrease in an age-dependent manner compared with wild-type females. Further analysis shows Meiok21-/- oocytes also have reduced rates of germinal vesicle breakdown and the first polar body extrusion when cultured in vitro, indicating poor oocyte quality. Additionally, Meiok21-/- oocytes have more chromosomes bearing a single distally localized crossover (chiasmata), suggesting a possible defect in crossover maturation. Taken together, our findings indicate critical roles for MEIOK21 in ensuring the number and quality of oocytes in the follicles.

Keywords

MEIOK21; fertility; meiosis; oocyte; recombination; synapsis.

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