2. Academic Validation
  3. Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes

Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes

  • Biochim Biophys Acta Mol Cell Res. 2022 Sep;1869(9):119306. doi: 10.1016/j.bbamcr.2022.119306.
Zhen-Yu She 1,  Meng-Fei Xu 2,  Sun-Ying Jiang 2,  Ya-Lan Wei 3
Affiliations

Affiliations

  • 1 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350122, China; Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, Fujian 350122, China. Electronic address: zhenyushe@fjmu.edu.cn.
  • 2 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350122, China; Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, Fujian 350122, China.
  • 3 Fujian Obstetrics and Gynecology Hospital, Fuzhou, Fujian 350011, China; Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350001, China.
PMID: 35680098 DOI: 10.1016/j.bbamcr.2022.119306
Abstract

Genome stability depends on chromosome congression and alignment during cell division. Kinesin-7 CENP-E is critical for kinetochore-microtubule attachment and chromosome alignment, which contribute to genome stability in mitosis. However, the functions and mechanisms of CENP-E in the meiotic division of male spermatocytes remain largely unknown. In this study, by combining the use of chemical inhibitors, siRNA-mediated gene knockdown, immunohistochemistry, and high-resolution microscopy, we have found that CENP-E inhibition results in chromosome misalignment and metaphase arrest in dividing spermatocyte during meiosis. Strikingly, we have revealed that CENP-E regulates spindle organization in metaphase I spermatocytes and cultured GC-2 spd cells. CENP-E depletion leads to spindle elongation, chromosome misalignment, and chromosome instability in spermatocytes. Together, these findings indicate that CENP-E mediates the kinetochore recruitment of BubR1, spindle assembly checkpoint and chromosome alignment in dividing spermatocytes, which finally contribute to faithful chromosome segregation and chromosome stability in the male meiotic division.

Keywords

CENP-E; Chromosome; Kinesin-7; Meiosis; Spermatocyte; Spindle.

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