N-acetylserotonin protects PC12 cells from hydrogen peroxide induced damage through ROS mediated PI3K / AKT pathway

N-acetylserotonin (NAS) exerts neuroprotective, antioxidant, and anti-apoptotic effects. Oxidative stress and Apoptosis are the primary causes of spinal cord injury (SCI). Herein, we explored potential protective effects and mechanisms of NAS in a neuron oxidative damage model in vitro. We established an oxidative damage model in PC12 cells induced by hydrogen peroxide (H₂O₂) and treated these cells with NAS. NAS enhanced the activity of superoxide dismutase and halted the increase in Reactive Oxygen Species (ROS) and the expression of inducible nitric oxide synthase. Additionally, NAS promoted protein expression of Bcl-2, but inhibited protein expressions of Fas, FADD, cytochrome c, Bax, cleaved caspase-9, and cleaved Caspase-3, namely, decreasing protein expression of the Fas and mitochondrial pathways. Furthermore, it reduced the rate of Apoptosis and necroptosis-related protein expressions of MLKL and p-MLKL. Moreover, NAS promoted the protein expression of p-PI3K and p-AKT, and the addition of the PI3K Inhibitor LY294002 partially attenuated the antioxidant stress and anti-apoptotic effects of NAS in H₂O₂ stimulated PC12 cells. In conclusion, NAS protected PC12 cells from Apoptosis and oxidative stress induced by H₂O₂ by inhibiting ROS activity and activating the PI3K/Akt signaling pathway.