2. Academic Validation
  3. Strongylocentrotus nudus egg polysaccharide (SEP) suppresses HBV replication via activation of TLR4-induced immune pathway

Strongylocentrotus nudus egg polysaccharide (SEP) suppresses HBV replication via activation of TLR4-induced immune pathway

  • Int J Biol Macromol. 2023 Jun 22;125539. doi: 10.1016/j.ijbiomac.2023.125539.
Haifei Yu 1,  Wanyu Deng 2,  Shuo Chen 1,  Bo Qin 3,  Yongxuan Yao 4,  Changlin Zhou 5,  Min Guo 6
Affiliations

Affiliations

  • 1 State Key Laboratory of Natural Medicines, School of Life Science & Technolgy, China Pharmaceutical University, Nanjing 211198, Jiangsu province, China.
  • 2 College of life science, Shangrao Normal University, Shangrao 334001, Jiangxi province, China.
  • 3 Shaoxing Women and Children's Hospital, Shaoxing 312000, Zhejiang, China.
  • 4 Guangzhou Institute of Pediatrics, Guangzhou Women and Children Medical Center, Guangzhou 510623, China. Electronic address: yaoyx@wh.iov.cn.
  • 5 State Key Laboratory of Natural Medicines, School of Life Science & Technolgy, China Pharmaceutical University, Nanjing 211198, Jiangsu province, China. Electronic address: cl_zhou@cpu.edu.cn.
  • 6 State Key Laboratory of Natural Medicines, School of Life Science & Technolgy, China Pharmaceutical University, Nanjing 211198, Jiangsu province, China. Electronic address: gm478220352@126.com.
PMID: 37355064 DOI: 10.1016/j.ijbiomac.2023.125539
Abstract

Chronic hepatitis B virus (HBV) Infection is a worldwide public health problem that causes significant liver-related morbidity and mortality. In our previous study, Strongylocentrotus nudus eggs polysaccharide (SEP), extracted from sea urchins, had immunomodulatory and antitumor effects. Whether SEP has anti-HBV activity is still obscure. This study demonstrated that SEP decreased the secretion of hepatitis B surface antigen (HBsAg) and e antigen (HBeAg), as well as the replication and transcription of HBV both in vitro and in vivo. Immunofluorescence and immunohistochemistry results showed that the level of HBV core antigen (HBcAg) was clearly reduced by SEP treatment. Mechanistically, RT-qPCR, western blot, and confocal microscopy analysis showed that SEP significantly increased the expression of Toll-like Receptor 4 (TLR4) and co-localization with TLR4. The downstream molecules of TLR4, including NF-κB and IRF3, were activated and the expression of IFN-β, TNF-α, IL-6, OAS, and MxA were also increased, which could suppress HBV replication. Moreover, SEP inhibited other genotypes of HBV and hepatitis C virus (HCV) replication in vitro. In summary, SEP could be investigated as a potential anti-HBV drug capable of modulating the innate immune.

Keywords

Hepatitis B virus; IFN-β; IRF3; NF-κb; Strongylocentrotus nudus egg polysaccharide; Toll-like receptor 4.

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